Cellular Respiration Rate: Yeast Metabolism and Q10 Temperature Coefficient
Overview
Quantifies cellular respiration and enzyme kinetics. Demonstrates temperature's effect on biochemical reactions.
Why Do This Experiment?
This experiment teaches key concepts in Biology.
Materials Needed
- •Yeast(1)
- •glucose(1)
- •water bath(1)
- •CO2 sensor or collection apparatus(1)
- •thermometer(1)
Step-by-Step Instructions
- 1
1. Set up yeast cultures at different temperatures (0��C, 20��C, 30��C, 40��C)
- 2
2. Measure CO2 production rate for each
- 3
3. Plot temperature vs respiration rate
- 4
4. Calculate Q10 value (rate doubling per 10��C increase)
The Science Behind It
Quantifies cellular respiration and enzyme kinetics. Demonstrates temperature's effect on biochemical reactions.
Learning Outcomes
- ✓Understand basic biology concepts
- ✓Develop observation skills
- ✓Learn through hands-on experimentation
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